6NUW
Yeast Ctf19 complex
Summary for 6NUW
| Entry DOI | 10.2210/pdb6nuw/pdb |
| EMDB information | 0523 |
| Descriptor | Inner kinetochore subunit IML3, Inner kinetochore subunit Mcm22, Inner kinetochore subunit Mcm16, ... (12 entities in total) |
| Functional Keywords | kinetochore, cell cycle |
| Biological source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) More |
| Total number of polymer chains | 13 |
| Total formula weight | 493093.15 |
| Authors | Hinshaw, S.M.,Harrison, S.C. (deposition date: 2019-02-03, release date: 2019-04-03, Last modification date: 2024-03-20) |
| Primary citation | Hinshaw, S.M.,Harrison, S.C. The structure of the Ctf19c/CCAN from budding yeast. Elife, 8:-, 2019 Cited by PubMed Abstract: Eukaryotic kinetochores connect spindlemicrotubules to chromosomal centromeres. A group of proteins called the Ctf19 complex (Ctf19c) in yeast and the constitutive centromere associated network (CCAN) in other organisms creates the foundation of a kinetochore. The Ctf19c/CCAN influences the timing of kinetochore assembly, sets its location by associating with a specialized nucleosome containing the histone H3 variant Cse4/CENP-A, and determines the organization of the microtubule attachment apparatus. We present here the structure of a reconstituted 13-subunit Ctf19c determined by cryo-electron microscopy at ~4 Å resolution. The structure accounts for known and inferred contacts with the Cse4 nucleosome and for an observed assembly hierarchy. We describe its implications for establishment of kinetochores and for their regulation by kinases throughout the cell cycle. PubMed: 30762520DOI: 10.7554/eLife.44239 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.25 Å) |
Structure validation
Download full validation report
