6NTS

Protein Phosphatase 2A (Aalpha-B56alpha-Calpha) holoenzyme in complex with a Small Molecule Activator of PP2A (SMAP)

6NTS の概要

• エントリーDOI: 10.2210/pdb6nts/pdb
• EMDBエントリー: 0510
• 分子名称: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, ... (5 entities in total)
• 機能のキーワード: holoenzyme complex, phosphatase, activator, hydrolase-activator complex, hydrolase/activator
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 157296.80

構造登録者

Huang, W.,Taylor, D. (登録日: 2019-01-30, 公開日: 2020-05-06, 最終更新日: 2025-06-04)

主引用文献

Leonard, D.,Huang, W.,Izadmehr, S.,O'Connor, C.M.,Wiredja, D.D.,Wang, Z.,Zaware, N.,Chen, Y.,Schlatzer, D.M.,Kiselar, J.,Vasireddi, N.,Schuchner, S.,Perl, A.L.,Galsky, M.D.,Xu, W.,Brautigan, D.L.,Ogris, E.,Taylor, D.J.,Narla, G.
Selective PP2A Enhancement through Biased Heterotrimer Stabilization.
Cell, 181:688-701.e16, 2020

PubMed Abstract: Impairment of protein phosphatases, including the family of serine/threonine phosphatases designated PP2A, is essential for the pathogenesis of many diseases, including cancer. The ability of PP2A to dephosphorylate hundreds of proteins is regulated by over 40 specificity-determining regulatory "B" subunits that compete for assembly and activation of heterogeneous PP2A heterotrimers. Here, we reveal how a small molecule, DT-061, specifically stabilizes the B56α-PP2A holoenzyme in a fully assembled, active state to dephosphorylate selective substrates, such as its well-known oncogenic target, c-Myc. Our 3.6 Å structure identifies molecular interactions between DT-061 and all three PP2A subunits that prevent dissociation of the active enzyme and highlight inherent mechanisms of PP2A complex assembly. Thus, our findings provide fundamental insights into PP2A complex assembly and regulation, identify a unique interfacial stabilizing mode of action for therapeutic targeting, and aid in the development of phosphatase-based therapeutics tailored against disease specific phospho-protein targets.

PubMed: 32315618
DOI: 10.1016/j.cell.2020.03.038

実験手法

ELECTRON MICROSCOPY (3.63 Å)