6NPL

Cryo-EM structure of NKCC1

6NPL の概要

• エントリーDOI: 10.2210/pdb6npl/pdb
• EMDBエントリー: 0473
• 分子名称: Solute carrier family 12 (sodium/potassium/chloride transporter), member 2, POTASSIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Danio rerio (Zebrafish)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 206827.43

構造登録者

Feng, L.,Liao, M.F.,Orlando, B.,Zhang, J.R. (登録日: 2019-01-17, 公開日: 2019-07-31, 最終更新日: 2024-10-30)

主引用文献

Chew, T.A.,Orlando, B.J.,Zhang, J.,Latorraca, N.R.,Wang, A.,Hollingsworth, S.A.,Chen, D.H.,Dror, R.O.,Liao, M.,Feng, L.
Structure and mechanism of the cation-chloride cotransporter NKCC1.
Nature, 572:488-492, 2019

PubMed Abstract: Cation-chloride cotransporters (CCCs) mediate the electroneutral transport of chloride, potassium and/or sodium across the membrane. They have critical roles in regulating cell volume, controlling ion absorption and secretion across epithelia, and maintaining intracellular chloride homeostasis. These transporters are primary targets for some of the most commonly prescribed drugs. Here we determined the cryo-electron microscopy structure of the Na-K-Cl cotransporter NKCC1, an extensively studied member of the CCC family, from Danio rerio. The structure defines the architecture of this protein family and reveals how cytosolic and transmembrane domains are strategically positioned for communication. Structural analyses, functional characterizations and computational studies reveal the ion-translocation pathway, ion-binding sites and key residues for transport activity. These results provide insights into ion selectivity, coupling and translocation, and establish a framework for understanding the physiological functions of CCCs and interpreting disease-related mutations.

PubMed: 31367042
DOI: 10.1038/s41586-019-1438-2

実験手法

ELECTRON MICROSCOPY (2.9 Å)