6NPE
C-abl Kinase domain with the activator(cmpd6), 2-cyano-N-(4-(3,4-dichlorophenyl)thiazol-2-yl)acetamide
Summary for 6NPE
| Entry DOI | 10.2210/pdb6npe/pdb |
| Descriptor | Tyrosine-protein kinase ABL1, SULFATE ION, 4-(4-METHYL-PIPERAZIN-1-YLMETHYL)-N-[4-METHYL-3-(4-PYRIDIN-3-YL-PYRIMIDIN-2-YLAMINO)-PHENYL]-BENZAMIDE, ... (6 entities in total) |
| Functional Keywords | activator, kinase, allosteric, signaling protein, transferase |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 2 |
| Total formula weight | 71827.43 |
| Authors | campobasso, N. (deposition date: 2019-01-17, release date: 2019-03-13, Last modification date: 2024-03-13) |
| Primary citation | Simpson, G.L.,Bertrand, S.M.,Borthwick, J.A.,Campobasso, N.,Chabanet, J.,Chen, S.,Coggins, J.,Cottom, J.,Christensen, S.B.,Dawson, H.C.,Evans, H.L.,Hobbs, A.N.,Hong, X.,Mangatt, B.,Munoz-Muriedas, J.,Oliff, A.,Qin, D.,Scott-Stevens, P.,Ward, P.,Washio, Y.,Yang, J.,Young, R.J. Identification and Optimization of Novel Small c-Abl Kinase Activators Using Fragment and HTS Methodologies. J. Med. Chem., 62:2154-2171, 2019 Cited by PubMed Abstract: Abelson kinase (c-Abl) is a ubiquitously expressed, nonreceptor tyrosine kinase which plays a key role in cell differentiation and survival. It was hypothesized that transient activation of c-Abl kinase via displacement of the N-terminal autoinhibitory "myristoyl latch", may lead to an increased hematopoietic stem cell differentiation. This would increase the numbers of circulating neutrophils and so be an effective treatment for chemotherapy-induced neutropenia. This paper describes the discovery and optimization of a thiazole series of novel small molecule c-Abl activators, initially identified by a high throughput screening. Subsequently, a scaffold-hop, which exploited the improved physicochemical properties of a dihydropyrazole analogue, identified through fragment screening, delivered potent, soluble, cell-active c-Abl activators, which demonstrated the intracellular activation of c-Abl in vivo. PubMed: 30689376DOI: 10.1021/acs.jmedchem.8b01872 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.15 Å) |
Structure validation
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