Crystal structure of the Thermus thermophilus 70S ribosome in complex with erythromycin and bound to mRNA and A-, P-, and E-site tRNAs at 2.85A resolution

This is a large structure.

Summary for 6ND6

Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (60 entities in total)
Functional Keywordschloramphenicol, erythromycin, competition, antibiotic, 70s ribosome, inhibition of translation, peptidyl transferase center, nascent peptide exit tunnel, ribosome, ribosome-antibiotic complex, ribosome/antibiotic
Biological sourceEscherichia coli
Total number of polymer chains112
Total molecular weight4572351.56
Svetlov, M.S.,Plessa, E.,Chen, C.-W.,Bougas, A.,Krokidis, M.G.,Dinos, G.P.,Polikanov, Y.S. (deposition date: 2018-12-13, release date: 2019-02-20, Last modification date: 2019-05-01)
Primary citation
Svetlov, M.S.,Plessa, E.,Chen, C.W.,Bougas, A.,Krokidis, M.G.,Dinos, G.P.,Polikanov, Y.S.
High-resolution crystal structures of ribosome-bound chloramphenicol and erythromycin provide the ultimate basis for their competition.
RNA, 25:600-606, 2019
PubMed: 30733327 (PDB entries with the same primary citation)
DOI: 10.1261/rna.069260.118
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.30511 0.8% 5.6% 4.7% 0.51MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report