6ND4

Conformational switches control early maturation of the eukaryotic small ribosomal subunit

6ND4 の概要

• エントリーDOI: 10.2210/pdb6nd4/pdb
• EMDBエントリー: 0441
• 分子名称: 5'ETS rRNA, Utp4, Utp1, ... (28 entities in total)
• 機能のキーワード: ribosome assembly, ribosome
• 由来する生物種: Saccharomyces cerevisiae BY4741 (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 30
• 化学式量合計: 1867663.28

構造登録者

Hunziker, M.,Barandun, J.,Klinge, S. (登録日: 2018-12-13, 公開日: 2019-06-19, 最終更新日: 2024-03-20)

主引用文献

Hunziker, M.,Barandun, J.,Buzovetsky, O.,Steckler, C.,Molina, H.,Klinge, S.
Conformational switches control early maturation of the eukaryotic small ribosomal subunit.
Elife, 8:-, 2019

PubMed Abstract: Eukaryotic ribosome biogenesis is initiated with the transcription of pre-ribosomal RNA at the 5' external transcribed spacer, which directs the early association of assembly factors but is absent from the mature ribosome. The subsequent co-transcriptional association of ribosome assembly factors with pre-ribosomal RNA results in the formation of the small subunit processome. Here we show that stable rRNA domains of the small ribosomal subunit can independently recruit their own biogenesis factors in vivo. The final assembly and compaction of the small subunit processome requires the presence of the 5' external transcribed spacer RNA and all ribosomal RNA domains. Additionally, our cryo-electron microscopy structure of the earliest nucleolar pre-ribosomal assembly - the 5' external transcribed spacer ribonucleoprotein - provides a mechanism for how conformational changes in multi-protein complexes can be employed to regulate the accessibility of binding sites and therefore define the chronology of maturation events during early stages of ribosome assembly.

PubMed: 31206356
DOI: 10.7554/eLife.45185

実験手法

ELECTRON MICROSCOPY (4.3 Å)