6NC9
Lipid II flippase MurJ, outward-facing conformation
Summary for 6NC9
| Entry DOI | 10.2210/pdb6nc9/pdb |
| Descriptor | Lipid II flippase MurJ, PENTAETHYLENE GLYCOL, (2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (6 entities in total) |
| Functional Keywords | transporter, flippase, peptidoglycan, cell wall, transport protein |
| Biological source | Thermosipho africanus (strain TCF52B) |
| Total number of polymer chains | 1 |
| Total formula weight | 59747.32 |
| Authors | Kuk, A.C.Y.,Lee, S.-Y. (deposition date: 2018-12-11, release date: 2019-04-17, Last modification date: 2023-10-11) |
| Primary citation | Kuk, A.C.Y.,Hao, A.,Guan, Z.,Lee, S.Y. Visualizing conformation transitions of the Lipid II flippase MurJ. Nat Commun, 10:1736-1736, 2019 Cited by PubMed Abstract: The biosynthesis of many polysaccharides, including bacterial peptidoglycan and eukaryotic N-linked glycans, requires transport of lipid-linked oligosaccharide (LLO) precursors across the membrane by specialized flippases. MurJ is the flippase for the lipid-linked peptidoglycan precursor Lipid II, a key player in bacterial cell wall synthesis, and a target of recently discovered antibacterials. However, the flipping mechanism of LLOs including Lipid II remains poorly understood due to a dearth of structural information. Here we report crystal structures of MurJ captured in inward-closed, inward-open, inward-occluded and outward-facing conformations. Together with mutagenesis studies, we elucidate the conformational transitions in MurJ that mediate lipid flipping, identify the key ion for function, and provide a framework for the development of inhibitors. PubMed: 30988294DOI: 10.1038/s41467-019-09658-0 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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