6N8L

Cryo-EM structure of early cytoplasmic-late (ECL) pre-60S ribosomal subunit

これはPDB形式変換不可エントリーです。

6N8L の概要

• エントリーDOI: 10.2210/pdb6n8l/pdb
• 関連するPDBエントリー: 6N8J 6N8K 6N8M 6N8N 6N8O
• EMDBエントリー: 0369 0370 0371 0372 0373 0374
• 分子名称: 60S ribosomal export protein NMD3, 60S ribosomal protein L6-A, 60S ribosomal protein L32, ... (48 entities in total)
• 機能のキーワード: ribosome biogenesis, nmd3, peptidyl transferase center, ribosome
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 2130492.34

構造登録者

Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W. (登録日: 2018-11-29, 公開日: 2019-03-13, 最終更新日: 2024-11-06)

主引用文献

Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W.
Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
Nat Commun, 10:958-958, 2019

PubMed Abstract: The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.

PubMed: 30814529
DOI: 10.1038/s41467-019-08880-0

実験手法

ELECTRON MICROSCOPY (3.6 Å)