6N6I

Human REXO2 bound to pGG

6N6I の概要

• エントリーDOI: 10.2210/pdb6n6i/pdb
• 分子名称: RNA exonuclease 2 homolog,Small fragment nuclease, RNA (5'-R(P*GP*G)-3'), GLYCEROL, ... (7 entities in total)
• 機能のキーワード: 3'-5' exoribonuclease, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49263.77

構造登録者

Lormand, J.D.,Sondermann, H. (登録日: 2018-11-26, 公開日: 2019-06-12, 最終更新日: 2023-10-11)

主引用文献

Kim, S.K.,Lormand, J.D.,Weiss, C.A.,Eger, K.A.,Turdiev, H.,Turdiev, A.,Winkler, W.C.,Sondermann, H.,Lee, V.T.
A dedicated diribonucleotidase resolves a key bottleneck for the terminal step of RNA degradation.
Elife, 8:-, 2019

PubMed Abstract: Degradation of RNA polymers, an ubiquitous process in all cells, is catalyzed by specific subsets of endo- and exoribonucleases that together recycle RNA fragments into nucleotide monophosphate. In γ-proteobacteria, 3-'5' exoribonucleases comprise up to eight distinct enzymes. Among them, Oligoribonuclease (Orn) is unique as its activity is required for clearing short RNA fragments, which is important for cellular fitness. However, the molecular basis of Orn's unique cellular function remained unclear. Here, we show that Orn exhibits exquisite substrate preference for diribonucleotides. Crystal structures of substrate-bound Orn reveal an active site optimized for diribonucleotides. While other cellular RNases process oligoribonucleotides down to diribonucleotide entities, Orn is the one and only diribonucleotidase that completes the terminal step of RNA degradation. Together, our studies indicate RNA degradation as a step-wise process with a dedicated enzyme for the clearance of a specific intermediate pool, diribonucleotides, that affects cellular physiology and viability.

PubMed: 31225796
DOI: 10.7554/eLife.46313

実験手法

X-RAY DIFFRACTION (1.431 Å)