6N1C

Crystal structure of Inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1

6N1C の概要

• エントリーDOI: 10.2210/pdb6n1c/pdb
• 分子名称: Inorganic pyrophosphatase, SODIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (6 entities in total)
• 機能のキーワード: ssgcid, structural genomics, seattle structural genomics center for infectious disease, hydrolase
• 由来する生物種: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 127948.99

構造登録者

Seattle Structural Genomics Center for Infectious Disease (SSGCID) (登録日: 2018-11-08, 公開日: 2018-12-19, 最終更新日: 2024-07-17)

主引用文献

Moorefield, J.,Konuk, Y.,Norman, J.O.,Abendroth, J.,Edwards, T.E.,Lorimer, D.D.,Mayclin, S.J.,Staker, B.L.,Craig, J.K.,Barett, K.F.,Barrett, L.K.,Van Voorhis, W.C.,Myler, P.J.,McLaughlin, K.J.
Characterization of a family I inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1.
Acta Crystallogr.,Sect.F, 79:257-266, 2023

PubMed Abstract: Inorganic pyrophosphate (PP) is generated as an intermediate or byproduct of many fundamental metabolic pathways, including DNA/RNA synthesis. The intracellular concentration of PP must be regulated as buildup can inhibit many critical cellular processes. Inorganic pyrophosphatases (PPases) hydrolyze PP into two orthophosphates (P), preventing the toxic accumulation of the PP byproduct in cells and making P available for use in biosynthetic pathways. Here, the crystal structure of a family I inorganic pyrophosphatase from Legionella pneumophila is reported at 2.0 Å resolution. L. pneumophila PPase (LpPPase) adopts a homohexameric assembly and shares the oligonucleotide/oligosaccharide-binding (OB) β-barrel core fold common to many other bacterial family I PPases. LpPPase demonstrated hydrolytic activity against a general substrate, with Mg being the preferred metal cofactor for catalysis. Legionnaires' disease is a severe respiratory infection caused primarily by L. pneumophila, and thus increased characterization of the L. pneumophila proteome is of interest.

PubMed: 37728609
DOI: 10.1107/S2053230X23008002

実験手法

X-RAY DIFFRACTION (2 Å)