6MZD

Human TFIID Lobe A canonical

6MZD の概要

• エントリーDOI: 10.2210/pdb6mzd/pdb
• EMDBエントリー: 9298 9299 9300 9301 9302 9305 9306
• 分子名称: Transcription initiation factor TFIID subunit 1, Transcription initiation factor TFIID subunit 13, TATA-box-binding protein, ... (12 entities in total)
• 機能のキーワード: transcription, dna, nuclear
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 737651.38

構造登録者

Patel, A.B.,Louder, R.K.,Greber, B.J.,Grunberg, S.,Luo, J.,Fang, J.,Liu, Y.,Ranish, J.,Hahn, S.,Nogales, E. (登録日: 2018-11-05, 公開日: 2018-11-28, 最終更新日: 2024-10-16)

主引用文献

Patel, A.B.,Louder, R.K.,Greber, B.J.,Grunberg, S.,Luo, J.,Fang, J.,Liu, Y.,Ranish, J.,Hahn, S.,Nogales, E.
Structure of human TFIID and mechanism of TBP loading onto promoter DNA.
Science, 362:-, 2018

PubMed Abstract: The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy, chemical cross-linking mass spectrometry, and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA box-binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.

PubMed: 30442764
DOI: 10.1126/science.aau8872

実験手法

ELECTRON MICROSCOPY (9.8 Å)