6MXF

MicroED structure of thiostrepton at 1.9 A resolution

6MXF の概要

• エントリーDOI: 10.2210/pdb6mxf/pdb
• EMDBエントリー: 9282 9283 9284 9285 9286 9287 9288 9289 9290 9291 9292
• 関連するBIRD辞書のPRD_ID: PRD_000223
• 分子名称: Thiostrepton (2 entities in total)
• 機能のキーワード: antibiotic
• 由来する生物種: Streptomyces azureus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1805.98

構造登録者

Jones, C.G.,Martynowycz, M.W.,Hattne, J.,Fulton, T.,Stoltz, B.M.,Rodriguez, J.A.,Nelson, H.M.,Gonen, T. (登録日: 2018-10-30, 公開日: 2018-11-21, 最終更新日: 2023-11-15)

主引用文献

Jones, C.G.,Martynowycz, M.W.,Hattne, J.,Fulton, T.J.,Stoltz, B.M.,Rodriguez, J.A.,Nelson, H.M.,Gonen, T.
The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination.
ACS Cent Sci, 4:1587-1592, 2018

PubMed Abstract: In the many scientific endeavors that are driven by organic chemistry, unambiguous identification of small molecules is of paramount importance. Over the past 50 years, NMR and other powerful spectroscopic techniques have been developed to address this challenge. While almost all of these techniques rely on inference of connectivity, the unambiguous determination of a small molecule's structure requires X-ray and/or neutron diffraction studies. In practice, however, X-ray crystallography is rarely applied in routine organic chemistry due to intrinsic limitations of both the analytes and the technique. Here we report the use of the electron cryo-microscopy (cryoEM) method microcrystal electron diffraction (MicroED) to provide routine and unambiguous structural determination of small organic molecules. From simple powders, with minimal sample preparation, we could collect high-quality MicroED data from nanocrystals (∼100 nm, ∼10 g) resulting in atomic resolution (<1 Å) crystal structures in minutes.

PubMed: 30555912
DOI: 10.1021/acscentsci.8b00760

実験手法

ELECTRON CRYSTALLOGRAPHY (1.91 Å)