Summary for 6MX8
| Entry DOI | 10.2210/pdb6mx8/pdb |
| Descriptor | ALK tyrosine kinase receptor, 5-chloro-N~4~-[2-(dimethylphosphoryl)phenyl]-N~2~-{2-methoxy-4-[4-(4-methylpiperazin-1-yl)piperidin-1-yl]phenyl}pyrimidine-2,4-diamine (3 entities in total) |
| Functional Keywords | alk kinase, brigatinib, tyrosine kinase, transferase |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 35073.80 |
| Authors | Dougan, D.R.,Zhou, T. (deposition date: 2018-10-30, release date: 2018-12-12, Last modification date: 2024-03-13) |
| Primary citation | Huang, W.S.,Liu, S.,Zou, D.,Thomas, M.,Wang, Y.,Zhou, T.,Romero, J.,Kohlmann, A.,Li, F.,Qi, J.,Cai, L.,Dwight, T.A.,Xu, Y.,Xu, R.,Dodd, R.,Toms, A.,Parillon, L.,Lu, X.,Anjum, R.,Zhang, S.,Wang, F.,Keats, J.,Wardwell, S.D.,Ning, Y.,Xu, Q.,Moran, L.E.,Mohemmad, Q.K.,Jang, H.G.,Clackson, T.,Narasimhan, N.I.,Rivera, V.M.,Zhu, X.,Dalgarno, D.,Shakespeare, W.C. Discovery of Brigatinib (AP26113), a Phosphine Oxide-Containing, Potent, Orally Active Inhibitor of Anaplastic Lymphoma Kinase. J. Med. Chem., 59:4948-4964, 2016 Cited by PubMed Abstract: In the treatment of echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase positive (ALK+) non-small-cell lung cancer (NSCLC), secondary mutations within the ALK kinase domain have emerged as a major resistance mechanism to both first- and second-generation ALK inhibitors. This report describes the design and synthesis of a series of 2,4-diarylaminopyrimidine-based potent and selective ALK inhibitors culminating in identification of the investigational clinical candidate brigatinib. A unique structural feature of brigatinib is a phosphine oxide, an overlooked but novel hydrogen-bond acceptor that drives potency and selectivity in addition to favorable ADME properties. Brigatinib displayed low nanomolar IC50s against native ALK and all tested clinically relevant ALK mutants in both enzyme-based biochemical and cell-based viability assays and demonstrated efficacy in multiple ALK+ xenografts in mice, including Karpas-299 (anaplastic large-cell lymphomas [ALCL]) and H3122 (NSCLC). Brigatinib represents the most clinically advanced phosphine oxide-containing drug candidate to date and is currently being evaluated in a global phase 2 registration trial. PubMed: 27144831DOI: 10.1021/acs.jmedchem.6b00306 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.96 Å) |
Structure validation
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