6MJB

Structure of Candida glabrata Csm1:Dsn1(14-72) complex

Summary for 6MJB

• Entry DOI: 10.2210/pdb6mjb/pdb
• Descriptor: Monopolin complex subunit CSM1, Kinetochore-associated protein DSN1 (3 entities in total)
• Functional Keywords: monopolin, kinetochore, cell cycle
• Biological source: Candida glabrata (Yeast); More
• Total number of polymer chains: 3
• Total formula weight: 33690.84

Authors

Singh, N.,Corbett, K.D. (deposition date: 2018-09-20, release date: 2018-11-07, Last modification date: 2024-11-06)

Primary citation

Plowman, R.,Singh, N.,Tromer, E.C.,Payan, A.,Duro, E.,Spanos, C.,Rappsilber, J.,Snel, B.,Kops, G.J.P.L.,Corbett, K.D.,Marston, A.L.
The molecular basis of monopolin recruitment to the kinetochore.
Chromosoma, 128:331-354, 2019

PubMed Abstract: The monopolin complex is a multifunctional molecular crosslinker, which in S. pombe binds and organises mitotic kinetochores to prevent aberrant kinetochore-microtubule interactions. In the budding yeast S. cerevisiae, whose kinetochores bind a single microtubule, the monopolin complex crosslinks and mono-orients sister kinetochores in meiosis I, enabling the biorientation and segregation of homologs. Here, we show that both the monopolin complex subunit Csm1 and its binding site on the kinetochore protein Dsn1 are broadly distributed throughout eukaryotes, suggesting a conserved role in kinetochore organisation and function. We find that budding yeast Csm1 binds two conserved motifs in Dsn1, one (termed Box 1) representing the ancestral, widely conserved monopolin binding motif and a second (termed Box 2-3) with a likely role in enforcing specificity of sister kinetochore crosslinking. We find that Box 1 and Box 2-3 bind the same conserved hydrophobic cavity on Csm1, suggesting competition or handoff between these motifs. Using structure-based mutants, we also find that both Box 1 and Box 2-3 are critical for monopolin function in meiosis. We identify two conserved serine residues in Box 2-3 that are phosphorylated in meiosis and whose mutation to aspartate stabilises Csm1-Dsn1 binding, suggesting that regulated phosphorylation of these residues may play a role in sister kinetochore crosslinking specificity. Overall, our results reveal the monopolin complex as a broadly conserved kinetochore organiser in eukaryotes, which budding yeast have co-opted to mediate sister kinetochore crosslinking through the addition of a second, regulatable monopolin binding interface.

PubMed: 31037469
DOI: 10.1007/s00412-019-00700-0

Experimental method

X-RAY DIFFRACTION (2.27 Å)