6MJB

Structure of Candida glabrata Csm1:Dsn1(14-72) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	71.632, 71.632, 122.258
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.542 - 2.270
R-factor	0.1978
Rwork	0.195
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4r
RMSD bond length	0.007
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.000	2.390
High resolution limit [Å]	2.270	2.270
Rmerge	0.116	1.125
Rmeas	0.128	1.241
Rpim	0.053	0.520
Number of reflections	16495	2420
<I/σ(I)>	14.3
Completeness [%]	100.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.45 M ammonium sulfate, 5% PEG3350, 0.1 M Bis-Tris, pH 5.5, cryoprotectant: 25% glycerol