6MID
Cryo-EM structure of the ZIKV virion in complex with Fab fragments of the potently neutralizing human monoclonal antibody ZIKV-195
Summary for 6MID
| Entry DOI | 10.2210/pdb6mid/pdb |
| EMDB information | 9131 |
| Descriptor | E protein, M protein, monoclonal antibody ZIKV-195 heavy chain, ... (5 entities in total) |
| Functional Keywords | virus, monoclonal antibody, complex, virus-immune system complex, virus/immune system |
| Biological source | Zika virus (isolate ZIKV/Human/French Polynesia/10087PF/2013) (ZIKV) More |
| Total number of polymer chains | 8 |
| Total formula weight | 215995.65 |
| Authors | Long, F.,Rossmann, M.G. (deposition date: 2018-09-19, release date: 2019-01-16, Last modification date: 2024-10-23) |
| Primary citation | Long, F.,Doyle, M.,Fernandez, E.,Miller, A.S.,Klose, T.,Sevvana, M.,Bryan, A.,Davidson, E.,Doranz, B.J.,Kuhn, R.J.,Diamond, M.S.,Crowe Jr., J.E.,Rossmann, M.G. Structural basis of a potent human monoclonal antibody against Zika virus targeting a quaternary epitope. Proc. Natl. Acad. Sci. U.S.A., 116:1591-1596, 2019 Cited by PubMed Abstract: Zika virus (ZIKV) is a major human pathogen and member of the genus in the Flaviviridae family. In contrast to most other insect-transmitted flaviviruses, ZIKV also can be transmitted sexually and from mother to fetus in humans. During recent outbreaks, ZIKV infections have been linked to microcephaly, congenital disease, and Guillain-Barré syndrome. Neutralizing antibodies have potential as therapeutic agents. We report here a 4-Å-resolution cryo-electron microscopy structure of the ZIKV virion in complex with Fab fragments of the potently neutralizing human monoclonal antibody ZIKV-195. The footprint of the ZIKV-195 Fab fragment expands across two adjacent envelope (E) protein protomers. ZIKV neutralization by this antibody is presumably accomplished by cross-linking the E proteins, which likely prevents formation of E protein trimers required for fusion of the viral and cellular membranes. A single dose of ZIKV-195 administered 5 days after virus inoculation showed marked protection against lethality in a stringent mouse model of infection. PubMed: 30642974DOI: 10.1073/pnas.1815432116 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4 Å) |
Structure validation
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