6MDR

Cryo-EM structure of the Ceru+32/GFP-17 protomer

6MDR の概要

• エントリーDOI: 10.2210/pdb6mdr/pdb
• EMDBエントリー: 9104
• 分子名称: Ceru+32, GFP-17 (2 entities in total)
• 機能のキーワード: supercharged protein assembly, electrostatic interactions, 16-mer, d4 symmetry, luminescent protein
• 由来する生物種: Aequorea victoria (Jellyfish); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 445211.50

構造登録者

Simon, A.J.,Zhou, Y.,Ramasubramani, V.,Glaser, J.,Pothukuchy, A.,Golihar, J.,Gerberich, J.C.,Leggere, J.C.,Morrow, B.R.,Jung, C.,Glotzer, S.C.,Taylor, D.W.,Ellington, A.D. (登録日: 2018-09-05, 公開日: 2019-01-23, 最終更新日: 2024-03-13)

主引用文献

Simon, A.J.,Zhou, Y.,Ramasubramani, V.,Glaser, J.,Pothukuchy, A.,Gollihar, J.,Gerberich, J.C.,Leggere, J.C.,Morrow, B.R.,Jung, C.,Glotzer, S.C.,Taylor, D.W.,Ellington, A.D.
Supercharging enables organized assembly of synthetic biomolecules.
Nat Chem, 11:204-212, 2019

PubMed Abstract: Symmetrical protein oligomers are ubiquitous in biological systems and perform key structural and regulatory functions. However, there are few methods for constructing such oligomers. Here we have engineered completely synthetic, symmetrical oligomers by combining pairs of oppositely supercharged variants of a normally monomeric model protein through a strategy we term 'supercharged protein assembly' (SuPrA). We show that supercharged variants of green fluorescent protein can assemble into a variety of architectures including a well-defined symmetrical 16-mer structure that we solved using cryo-electron microscopy at 3.47 Å resolution. The 16-mer is composed of two stacked rings of octamers, in which the octamers contain supercharged proteins of alternating charges, and interactions within and between the rings are mediated by a variety of specific electrostatic contacts. The ready assembly of this structure suggests that combining oppositely supercharged pairs of protein variants may provide broad opportunities for generating novel architectures via otherwise unprogrammed interactions.

PubMed: 30643229
DOI: 10.1038/s41557-018-0196-3

実験手法

ELECTRON MICROSCOPY (3.47 Å)