6M6O

NMR SOLUTION STRUCTURE OF A C-FLIPs

Summary for 6M6O

• Entry DOI: 10.2210/pdb6m6o/pdb
• Descriptor: CASP8 and FADD-like apoptosis regulator (1 entity in total)
• Functional Keywords: apoptosis, c-flips
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 21588.22

Authors

Bai, Z.Q.,Hu, K.F. (deposition date: 2020-03-16, release date: 2021-03-17, Last modification date: 2024-05-15)

Primary citation

Bai, Z.Q.,Ma, X.,Liu, B.,Huang, T.,Hu, K.
Solution structure of c-FLIP death effector domains.
Biochem.Biophys.Res.Commun., 617:1-6, 2022

PubMed Abstract: The formation of death-inducing signaling complex (DISC) and death effector domain (DED) filament initiates extrinsic apoptosis. Recruitment and activation of procaspase-8 at the DISC are regulated by c-FLIP. The interaction between c-FLIP and procaspase-8 is mediated by their tandem DEDs (tDED). However, the structure of c-FLIP and how c-FLIP interferes with procaspase-8 activation at the DISC remain elusive. Here, we solved the monomeric structure of c-FLIP (F114G) at near physiological pH by solution nuclear magnetic resonance (NMR). Structural superimposition reveals c-FLIP (F114G) adopts a structural topology similar to that of procaspase-8. Our results provide a structural basis for understanding how c-FLIP interacts with procaspase-8 and the molecular mechanisms of c-FLIP in regulating cell death.

PubMed: 35688044
DOI: 10.1016/j.bbrc.2022.05.086

Experimental method

SOLUTION NMR