6M0W

Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM

Summary for 6M0W

• Entry DOI: 10.2210/pdb6m0w/pdb
• Descriptor: RNA (72-MER), DNA (28-MER), DNA (5'-D(*AP*AP*AP*GP*AP*AP*GP*C)-3'), ... (6 entities in total)
• Functional Keywords: crispr, hydrolase-dna-rna complex, hydrolase/dna/rna
• Biological source: Streptococcus thermophilus LMD-9; More
• Total number of polymer chains: 4
• Total formula weight: 163961.41

Authors

Zhang, Y.,Zhang, H.,Xu, X.,Wang, Y.,Chen, W.,Wang, Y.,Wu, Z.,Tang, N.,Wang, Y.,Zhao, S.,Gan, J.,Ji, Q. (deposition date: 2020-02-23, release date: 2020-09-09, Last modification date: 2023-11-29)

• Primary citation: Zhang, Y.,Zhang, H.,Xu, X.,Wang, Y.,Chen, W.,Wang, Y.,Wu, Z.,Tang, N.,Wang, Y.,Zhao, S.,Gan, J.,Ji, Q.; Catalytic-state structure and engineering of Streptococcus thermophilus Cas9; Nat Catal, 2020; DOI: 10.1038/s41929-020-00506-9

Experimental method

X-RAY DIFFRACTION (2.76 Å)