6LOF
Crystal structure of ZsYellow soaked by Cu2+
Summary for 6LOF
| Entry DOI | 10.2210/pdb6lof/pdb |
| Descriptor | GFP-like fluorescent chromoprotein FP538 (3 entities in total) |
| Functional Keywords | zsyellow, fluorescent protein, yellow |
| Biological source | Zoanthus sp. (Green polyp) More |
| Total number of polymer chains | 4 |
| Total formula weight | 52868.81 |
| Authors | Nam, K.H. (deposition date: 2020-01-05, release date: 2020-01-22, Last modification date: 2023-11-29) |
| Primary citation | Kim, I.J.,Xu, Y.,Nam, K.H. Spectroscopic and Structural Analysis of Cu 2+ -Induced Fluorescence Quenching of ZsYellow. Biosensors (Basel), 10:-, 2020 Cited by PubMed Abstract: Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each fluorescent protein exhibits unique spectroscopic properties and mechanisms for fluorescence quenching by metals. Therefore, the metal-induced fluorescence quenching analysis of various new fluorescent proteins would be important step towards the development of such fluorescent protein-based metal biosensors. Here, we first report the spectroscopic and structural analysis of the yellow fluorescent protein ZsYellow, following its metal-induced quenching. Spectroscopic analysis showed that ZsYellow exhibited a high degree of fluorescence quenching by Cu. During Cu-induced ZsYellow quenching, fluorescence emission was recovered by adding EDTA. The crystal structure of ZsYellow soaked in Cu solution was determined at a 2.6 Å resolution. The electron density map did not indicate the presence of Cu around the chromophore or the β-barrel surface, which resulted in fluorescence quenching without Cu binding to specific site in ZsYellow. Based on these results, we propose the fluorescence quenching to occur in a distance-dependent manner between the metal and the fluorescent protein, when these components get to a closer vicinity at higher metal concentrations. Our results provide useful insights for future development of fluorescent protein-based metal biosensors. PubMed: 32210006DOI: 10.3390/bios10030029 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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