6LLB

Crystal structure of mpy-RNase J (mutant S247A), an archaeal RNase J from Methanolobus psychrophilus R15, in complex with 6 nt RNA

6LLB の概要

• エントリーDOI: 10.2210/pdb6llb/pdb
• 分子名称: MPY-RNase J, RNA (5'-R(P*AP*AP*AP*AP*AP*A)-3'), ZINC ION, ... (5 entities in total)
• 機能のキーワード: exoribonuclease, beta-casp, mbl domain, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Methanolobus psychrophilus R15; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 109315.66

構造登録者

Li, D.F.,Hou, Y.J.,Guo, L. (登録日: 2019-12-22, 公開日: 2020-01-01, 最終更新日: 2023-11-22)

主引用文献

Li, J.,Hou, Y.,Gu, X.,Yue, L.,Guo, L.,Li, D.,Dong, X.
A newly identified duplex RNA unwinding activity of archaeal RNase J depends on processive exoribonucleolysis coupled steric occlusion by its structural archaeal loops.
Rna Biol., 17:1480-1491, 2020

PubMed Abstract: RNase J is a prokaryotic 5'-3' exo/endoribonuclease that functions in mRNA decay and rRNA maturation. Here, we report a novel duplex unwinding activity of mpy-RNase J, an archaeal RNase J from , which enables it to degrade duplex RNAs with hairpins up to 40 bp when linking a 5' single-stranded overhangs of ≥ 7 nt, corresponding to the RNA channel length. A 6-nt RNA-mpy-RNase J-S247A structure reveals the RNA-interacting residues and a steric barrier at the RNA channel entrance comprising two archaeal loops and two helices. Mutagenesis of the residues key to either exoribonucleolysis or RNA translocation diminished the duplex unwinding activity. Substitution of the residues in the steric barrier yielded stalled degradation intermediates at the duplex RNA regions. Thus, an exoribonucleolysis-driven and steric occlusion-based duplex unwinding mechanism was identified. The duplex unwinding activity confers mpy-RNase J the capability of degrading highly structured RNAs, including the bacterial REP RNA, and archaeal mRNAs, rRNAs, tRNAs, SRPs, RNase P and CD-box RNAs, providing an indicative of the potential key roles of mpy-RNase J in pleiotropic RNA metabolisms. Hydrolysis-coupled duplex unwinding activity was also detected in a bacterial RNase J, which may use a shared but slightly different unwinding mechanism from archaeal RNase Js, indicating that duplex unwinding is a common property of the prokaryotic RNase Js.

PubMed: 32552320
DOI: 10.1080/15476286.2020.1777379

実験手法

X-RAY DIFFRACTION (2.6 Å)