6LIR

crystal structure of chicken TCR for 2.0

Summary for 6LIR

• Entry DOI: 10.2210/pdb6lir/pdb
• Descriptor: TCR alpha chain, TCR beta chain (3 entities in total)
• Functional Keywords: tcr, chicken, immune system
• Biological source: Gallus gallus; More
• Total number of polymer chains: 4
• Total formula weight: 101176.96

Authors

Zhang, L.,Xia, C. (deposition date: 2019-12-12, release date: 2020-11-18, Last modification date: 2024-10-16)

Primary citation

Zhang, L.,Liu, Y.,Meng, G.,Liang, R.,Zhang, B.,Xia, C.
Structural and Biophysical Insights into the TCR alpha beta Complex in Chickens.
Iscience, 23:101828-101828, 2020

PubMed Abstract: In this work, chicken HPAIV H5N1 epitope-specific TCRαβ (ch-TCRαβ) was isolated and its structure was determined. The Cα domain of ch-TCRαβ does not exhibit the typical structure of human TCRαβ, and the DE loop extends outward, resulting in close proximity between the Cα domain of ch-TCRαβ and CD3εδ/γ. The FG loop of the Cβ domain of ch-TCRαβ is shorter. The changes in the C domains of ch-TCRαβ and the difference in chicken CD3εδ/γ confirm that the complexes formed by TCRαβ and CD3εδ/γ differ from those in humans. In the chicken complex, a positively charged cleft is formed between the two CDR3 loops that might accommodate the acidic side chains of the chicken pMHC-I-bound HPAIV epitope intermediate portion oriented toward ch-TCRαβ. This is the first reported structure of chicken TCRαβ, and it provides a structural model of the ancestral TCR system in the immune synapses between T cells and antigen-presenting cells in lower vertebrates.

PubMed: 33305184
DOI: 10.1016/j.isci.2020.101828

Experimental method

X-RAY DIFFRACTION (2.091 Å)