6LE3

Crystal structure of gluconate 5-dehydrogenase from Lentibacter algarum

6LE3 の概要

• エントリーDOI: 10.2210/pdb6le3/pdb
• 分子名称: Gluconate 5-dehydrogenase, ACETYL GROUP (3 entities in total)
• 機能のキーワード: gluconate 5-dehydrogenase, biosynthetic protein
• 由来する生物種: Lentibacter algarum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 108894.86

構造登録者

Yuan, H. (登録日: 2019-11-23, 公開日: 2020-05-13, 最終更新日: 2023-11-22)

主引用文献

Tian, D.,Fu, X.,Cao, W.,Yuan, H.
Crystal structure of gluconate 5-dehydrogenase from Lentibacter algarum.
Acta Crystallogr.,Sect.F, 76:228-234, 2020

PubMed Abstract: Gluconate 5-dehydrogenase (Ga5DH; EC 1.1.1.69) from Lentibacter algarum (LaGa5DH) was recombinantly expressed in Escherichia coli and purified to homogeneity. The protein was crystallized and the crystal structure was solved at 2.1 Å resolution. The crystal belonged to the monoclinic system, with space group P1 and unit-cell parameters a = 55.42, b = 55.48, c = 79.16 Å, α = 100.51, β = 105.66, γ = 97.99°. The structure revealed LaGaDH to be a tetramer, with each subunit consisting of six α-helices and three antiparallel β-hairpins. LaGa5DH has high structural similarity to other Ga5DH proteins, demonstrating that this enzyme is highly conserved.

PubMed: 32356525
DOI: 10.1107/S2053230X20005336

実験手法

X-RAY DIFFRACTION (2.1 Å)