6LC1

Structural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter

6LC1 の概要

• エントリーDOI: 10.2210/pdb6lc1/pdb
• 分子名称: DNA, Nuclear receptor subfamily 4 group A member 1, DNA (25-MER), ... (4 entities in total)
• 機能のキーワード: nuclear receptor, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 72068.73

構造登録者

Jiang, L.,Chen, Y. (登録日: 2019-11-16, 公開日: 2019-12-18, 最終更新日: 2023-11-22)

主引用文献

Jiang, L.,Wei, H.,Yan, N.,Dai, S.,Li, J.,Qu, L.,Chen, X.,Guo, M.,Chen, Z.,Chen, Y.
Structural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter.
Biochem.Biophys.Res.Commun., 523:1-5, 2020

PubMed Abstract: The nuclear receptor NR4A subfamily (NR4A1/NGFI-B, NR4A2/Nurr1 and NR4A3/NOR-1) can recognize different classes of DNA response elements either as a monomer, homodimer, or heterodimer. In this study, we determined the structure of the NR4A1 DNA-binding domain (NR4A1-DBD) bound to natural Nur-responsive elements (NurREs) in the promoter region of the pituitary proopiomelanocortin (POMC) gene (NurRE) at 3.12 Å resolution. The NR4A1-DBD molecules bound independently to this element in our structure. The N-terminal helix H1 forms specific contacts with major groove, and C-terminal extension interact extensively with minor groove. Moreover our modelling structure of NR4A1 large fragment complexed with NurRE indicated that ligand binding domain of NR4A might form dimer interactions to help recognize DNA. Overall, our analyses provide a molecular basis for DNA binding of NR4A proteins as a homodimer and novel insight into the molecular functions of NR4A modulation of gene expression.

PubMed: 31822342
DOI: 10.1016/j.bbrc.2019.11.192

実験手法

X-RAY DIFFRACTION (3.12 Å)