6LAS

the wildtype SAM-VI riboswitch bound to SAM

6LAS の概要

• エントリーDOI: 10.2210/pdb6las/pdb
• 分子名称: RNA (55-MER), U1 small nuclear ribonucleoprotein A, S-ADENOSYLMETHIONINE, ... (4 entities in total)
• 機能のキーワード: riboswitch, sam, sam-vi, rna, rna binding protein-rna complex
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 69222.75

構造登録者

Ren, A.,Sun, A. (登録日: 2019-11-13, 公開日: 2020-01-01, 最終更新日: 2024-10-30)

主引用文献

Sun, A.,Gasser, C.,Li, F.,Chen, H.,Mair, S.,Krasheninina, O.,Micura, R.,Ren, A.
SAM-VI riboswitch structure and signature for ligand discrimination.
Nat Commun, 10:5728-5728, 2019

PubMed Abstract: Riboswitches are metabolite-sensing, conserved domains located in non-coding regions of mRNA that are central to regulation of gene expression. Here we report the first three-dimensional structure of the recently discovered S-adenosyl-L-methionine responsive SAM-VI riboswitch. SAM-VI adopts a unique fold and ligand pocket that are distinct from all other known SAM riboswitch classes. The ligand binds to the junctional region with its adenine tightly intercalated and Hoogsteen base-paired. Furthermore, we reveal the ligand discrimination mode of SAM-VI by additional X-ray structures of this riboswitch bound to S-adenosyl-L-homocysteine and a synthetic ligand mimic, in combination with isothermal titration calorimetry and fluorescence spectroscopy to explore binding thermodynamics and kinetics. The structure is further evaluated by analysis of ligand binding to SAM-VI mutants. It thus provides a thorough basis for developing synthetic SAM cofactors for applications in chemical and synthetic RNA biology.

PubMed: 31844059
DOI: 10.1038/s41467-019-13600-9

実験手法

X-RAY DIFFRACTION (2.708 Å)