6LA8

349 bp di-nucleosome harboring cohesive DNA termini assembled with linker histone H1.0

Summary for 6LA8

• Entry DOI: 10.2210/pdb6la8/pdb
• Descriptor: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (9 entities in total)
• Functional Keywords: nucleosome, dna-protein complex, dna binding protein, dna binding protein-dna complex, linker histone, h1.0
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 19
• Total formula weight: 457416.16

Authors

Adhireksan, Z.,Lee, P.L.,Sharma, D.,Davey, C.A. (deposition date: 2019-11-12, release date: 2020-10-07, Last modification date: 2023-11-22)

Primary citation

Adhireksan, Z.,Sharma, D.,Lee, P.L.,Davey, C.A.
Near-atomic resolution structures of interdigitated nucleosome fibres.
Nat Commun, 11:4747-4747, 2020

PubMed Abstract: Chromosome structure at the multi-nucleosomal level has remained ambiguous in spite of its central role in epigenetic regulation and genome dynamics. Recent investigations of chromatin architecture portray diverse modes of interaction within and between nucleosome chains, but how this is realized at the atomic level is unclear. Here we present near-atomic resolution crystal structures of nucleosome fibres that assemble from cohesive-ended dinucleosomes with and without linker histone. As opposed to adopting folded helical '30 nm' structures, the fibres instead assume open zigzag conformations that are interdigitated with one another. Zigzag conformations obviate extreme bending of the linker DNA, while linker DNA size (nucleosome repeat length) dictates fibre configuration and thus fibre-fibre packing, which is supported by variable linker histone binding. This suggests that nucleosome chains have a predisposition to interdigitate with specific characteristics under condensing conditions, which rationalizes observations of local chromosome architecture and the general heterogeneity of chromatin structure.

PubMed: 32958761
DOI: 10.1038/s41467-020-18533-2

Experimental method

X-RAY DIFFRACTION (3.4 Å)