6L6R

Crystal structure of LRP6 E1E2-SOST complex

6L6R の概要

• エントリーDOI: 10.2210/pdb6l6r/pdb
• 分子名称: Low-density lipoprotein receptor-related protein 6, Sclerostin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: lrp6, sclerostin, wnt signaling, inhibitor, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 179362.10

構造登録者

Choi, H.-J.,Kim, J. (登録日: 2019-10-29, 公開日: 2020-10-28, 最終更新日: 2024-11-13)

主引用文献

Kim, J.,Han, W.,Park, T.,Kim, E.J.,Bang, I.,Lee, H.S.,Jeong, Y.,Roh, K.,Kim, J.,Kim, J.S.,Kang, C.,Seok, C.,Han, J.-K.,Choi, H.-J.
Sclerostin inhibits Wnt signaling through tandem interaction with two LRP6 ectodomains.
Nat Commun, 11:5357-5357, 2020

PubMed Abstract: Low-density lipoprotein receptor-related protein 6 (LRP6) is a coreceptor of the β-catenin-dependent Wnt signaling pathway. The LRP6 ectodomain binds Wnt proteins, as well as Wnt inhibitors such as sclerostin (SOST), which negatively regulates Wnt signaling in osteocytes. Although LRP6 ectodomain 1 (E1) is known to interact with SOST, several unresolved questions remain, such as the reason why SOST binds to LRP6 E1E2 with higher affinity than to the E1 domain alone. Here, we present the crystal structure of the LRP6 E1E2-SOST complex with two interaction sites in tandem. The unexpected additional binding site was identified between the C-terminus of SOST and the LRP6 E2 domain. This interaction was confirmed by in vitro binding and cell-based signaling assays. Its functional significance was further demonstrated in vivo using Xenopus laevis embryos. Our results provide insights into the inhibitory mechanism of SOST on Wnt signaling.

PubMed: 33097721
DOI: 10.1038/s41467-020-19155-4

実験手法

X-RAY DIFFRACTION (3.8 Å)