6KY4

Crystal structure of Sulfiredoxin from Arabidopsis thaliana

6KY4 の概要

• エントリーDOI: 10.2210/pdb6ky4/pdb
• 分子名称: Sulfiredoxin, chloroplastic/mitochondrial, ADENOSINE-5'-DIPHOSPHATE, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: sulfiredoxin, cysteine, sulfinic acid, peroxiredoxin repair protein, arabidopsis thaliana, antioxidant, oxidoreductase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13386.96

構造登録者

Liu, M.,Wang, J.,Li, X.,Li, M.,Sylvanno, M.J.,Zhang, M.,Wang, M. (登録日: 2019-09-16, 公開日: 2019-10-16, 最終更新日: 2023-11-22)

主引用文献

Liu, M.,Wang, J.,Li, X.,Sylvanno, M.J.,Li, M.,Zhang, M.,Wang, M.
The crystal structure of sulfiredoxin from Arabidopsis thaliana revealed a more robust antioxidant mechanism in plants.
Biochem.Biophys.Res.Commun., 520:347-352, 2019

PubMed Abstract: Typical 2-cysteine peroxiredoxins (2-Cys Prxs) are critical peroxidase sensors and could be deactivated by the hyperoxidation under oxidative stress. In plants, 2-Cys Prxs present at a high level in chloroplasts and are repaired by Sulfiredoxin. Whereas many studies have explored the mechanism of Sulfiredoxin from Homo sapiens (HsSrx), the molecular mechanism of Sulfiredoxin in plants with unique photosynthesis remains unclear. Here we report the crystal structure of Sulfiredoxin from Arabidopsis thaliana (AtSrx), which displayed a typical ParB/Srx fold with an ATP bound at a conservative nucleotide binding motif GCHR. Both the ADP binding pocket and the putative AtSrx-AtPrxA interaction surface of AtSrx are more positively charged comparing to HsSrx, suggesting a robust mechanism of AtSrx. These features illustrate the unique mechanisms of AtSrx, which are vital for figure out the strategies of plants to cope with oxidation stress.

PubMed: 31604522
DOI: 10.1016/j.bbrc.2019.10.034

実験手法

X-RAY DIFFRACTION (3.2 Å)