6KVK

Crystal structure of UDP-Sm-SrUGT76G1

6KVK の概要

• エントリーDOI: 10.2210/pdb6kvk/pdb
• 分子名称: UDP-glycosyltransferase 76G1, URIDINE-5'-DIPHOSPHATE, Steviolmonoside, ... (4 entities in total)
• 機能のキーワード: glycosyltransferase, diterpenoid, stevia rebaudiana, natural sweeteners, steviol glycosides (sgs)., transferase
• 由来する生物種: Stevia rebaudiana (Stevia)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53291.21

構造登録者

Li, J.X.,Liu, Z.F.,Wang, Y.,Zhang, P. (登録日: 2019-09-04, 公開日: 2019-11-20, 最終更新日: 2023-11-22)

主引用文献

Liu, Z.,Li, J.,Sun, Y.,Zhang, P.,Wang, Y.
Structural Insights into the Catalytic Mechanism of a Plant Diterpene Glycosyltransferase SrUGT76G1.
Plant Commun., 1:100004-100004, 2020

PubMed Abstract: Diterpene glycosyltransferase UGT76G1 from (SrUGT76G1) is key to the generation of economically important steviol glycosides (SGs), a group of natural sweeteners with high-intensity sweetness. SrUGT76G1 accommodates a wide range of steviol-derived substrates and many other small molecules. We report here the crystal structures of SrUGT76G1 in complex with multiple ligands to answer how this enzyme recognizes diterpenoid aglycones and catalyzes the 1,3-sugar chain branching. A spacious pocket for sugar-acceptor binding was observed from the determined SrUGT76G1 structures, which can explain its broad substrate spectrum. Residues Gly87 and Leu204 lining the pocket play key roles in switching between diterpenoid and flavonoid glucosylation. An engineered mutant of SrUGT76G1, T284S, could catalyze a selectively increased production of next-generation sweetener rebaudioside M, with diminished side product of rebaudioside I. Taken together, these resutls provide significant insights into molecular basis of the substrate specificity of scarcely documented diterpenoid glycosyltransferases and would facilitate the structure-guided glycoengineering to produce diversified diterpenoids with new activities.

PubMed: 33404544
DOI: 10.1016/j.xplc.2019.100004

実験手法

X-RAY DIFFRACTION (2.397 Å)