6KQB

A long chain secondary alcohol dehydrogenase of Micrococcus luteus

6KQB の概要

• エントリーDOI: 10.2210/pdb6kqb/pdb
• 関連するPDBエントリー: 6KQ9
• 分子名称: 3-hydroxybutyryl-CoA dehydrogenase (2 entities in total)
• 機能のキーワード: alcohol dehydrogenase, oxidoreductase
• 由来する生物種: Micrococcus luteus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73715.62

構造登録者

Kim, H.J.,Kim, J.S. (登録日: 2019-08-16, 公開日: 2020-08-19, 最終更新日: 2023-11-22)

主引用文献

Seo, E.J.,Kim, H.J.,Kim, M.J.,Kim, J.S.,Park, J.B.
Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from Micrococcus luteus for redox-neutral biotransformation of fatty acids.
Chem.Commun.(Camb.), 55:14462-14465, 2019

PubMed Abstract: Structure-based engineering of a NAD+-dependent secondary alcohol dehydrogenase from Micrococcus luteus led to a 1800-fold increase in catalytic efficiency for NADP+. Furthermore, the engineered enzymes (e.g., D37S/A38R/V39S/T15I) were successfully coupled to a NADPH-dependent Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 for redox-neutral biotransformations of C18 fatty acids into C9 chemicals.

PubMed: 31728457
DOI: 10.1039/c9cc06447h

実験手法

X-RAY DIFFRACTION (2.261 Å)