6KLQ

Tropomyosin of cardiac thin filament in high-calcium state

Summary for 6KLQ

• Entry DOI: 10.2210/pdb6klq/pdb
• EMDB information: 0711 0712 0714 0715 0717 0718 0797 0799
• Descriptor: Tropomyosin (1 entity in total)
• Functional Keywords: cardiac thin filament, contractile protein
• Biological source: Mus musculus
• Total number of polymer chains: 2
• Total formula weight: 23014.35

Authors

Oda, T.,Yanagisawa, H.,Wakabayashi, T. (deposition date: 2019-07-30, release date: 2020-01-15, Last modification date: 2024-03-27)

Primary citation

Oda, T.,Yanagisawa, H.,Wakabayashi, T.
Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.
J.Struct.Biol., 209:107450-107450, 2020

PubMed Abstract: Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.

PubMed: 31954841
DOI: 10.1016/j.jsb.2020.107450

Experimental method

ELECTRON MICROSCOPY (7.7 Å)