6KJ2

200kV MicroED structure of FUS (37-42) SYSGYS solved from single crystal at 0.67 A

Summary for 6KJ2

• Entry DOI: 10.2210/pdb6kj2/pdb
• EMDB information: 0697
• Descriptor: RNA-binding protein FUS (2 entities in total)
• Functional Keywords: fus, microed, ultrahigh resolution, rna binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 662.65

Authors

Zhou, H.,Luo, F.,Luo, Z.,Li, D.,Liu, C.,Li, X. (deposition date: 2019-07-20, release date: 2019-10-02, Last modification date: 2024-03-27)

Primary citation

Zhou, H.,Luo, F.,Luo, Z.,Li, D.,Liu, C.,Li, X.
Programming Conventional Electron Microscopes for Solving Ultrahigh-Resolution Structures of Small and Macro-Molecules.
Anal.Chem., 91:10996-11003, 2019

PubMed Abstract: Microcrystal electron diffraction (MicroED) is becoming a powerful tool in determining the crystal structures of biological macromolecules and small organic compounds. However, wide applications of this technique are still limited by the special requirement for radiation-tolerated movie-mode camera and the lack of automated data collection methods. Herein, we develop a stage-camera synchronization scheme to minimize the hardware requirements and enable the use of the conventional electron cryo-microscope with a single-frame CCD camera, which ensures not only the acquisition of ultrahigh-resolution diffraction data but also low cost in practice. This method renders the structure determination of both peptide and small organic compounds at ultrahigh resolution up to ∼0.60 Å with unambiguous assignment of nearly all hydrogen atoms. The present work provides a widely applicable solution for routine structure determination of MicroED and demonstrates the capability of the low-end 120 kV microscope with a CCD camera in solving ultrahigh resolution structures of both organic compounds and biological macromolecules.

PubMed: 31334636
DOI: 10.1021/acs.analchem.9b01162

Experimental method

ELECTRON CRYSTALLOGRAPHY (0.67 Å)