6K57

Crystal structure of dCas9 in complex with sgRNA and DNA (CGA PAM)

Summary for 6K57

• Entry DOI: 10.2210/pdb6k57/pdb
• Descriptor: sgRNA, CRISPR-associated endonuclease Cas9, target DNA, ... (5 entities in total)
• Functional Keywords: endonuclease, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 4
• Total formula weight: 197655.79

Authors

Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q. (deposition date: 2019-05-28, release date: 2019-09-25, Last modification date: 2023-11-22)

• Primary citation: Chen, W.,Zhang, H.,Zhang, Y.,Wang, Y.,Gan, J.,Ji, Q.; Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease.; Plos Biol., 17:e3000496-e3000496, 2019; PubMed: 31603896; DOI: 10.1371/journal.pbio.3000496

Experimental method

X-RAY DIFFRACTION (2.98 Å)