6K3H

Crystallographic Analysis of Nucleoside Diphosphate Kinase (NDK) from Aspergillus Flavus

Summary for 6K3H

• Entry DOI: 10.2210/pdb6k3h/pdb
• Descriptor: Nucleoside diphosphate kinase (1 entity in total)
• Functional Keywords: nucleoside diphosphate kinase, aspergillus flavus, filamentous fungi, transferase
• Biological source: Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167)
• Total number of polymer chains: 24
• Total formula weight: 412480.22

Authors

Wang, Y.,Wang, S.,Wang, S.H. (deposition date: 2019-05-18, release date: 2019-07-03, Last modification date: 2023-11-22)

Primary citation

Wang, Y.,Wang, S.,Nie, X.,Yang, K.,Xu, P.,Wang, X.,Liu, M.,Yang, Y.,Chen, Z.,Wang, S.
Molecular and structural basis of nucleoside diphosphate kinase-mediated regulation of spore and sclerotia development in the fungusAspergillus flavus.
J.Biol.Chem., 294:12415-12431, 2019

PubMed Abstract: The fundamental biological function of nucleoside diphosphate kinase (NDK) is to catalyze the reversible exchange of the γ-phosphate between nucleoside triphosphate (NTP) and nucleoside diphosphate (NDP). This kinase also has functions that extend beyond its canonically defined enzymatic role as a phosphotransferase. However, the role of NDK in filamentous fungi, especially in (), is not yet known. Here we report that has two NDK-encoding gene copies as assessed by qPCR. Using gene-knockout and complementation experiments, we found that AfNDK regulates spore and sclerotia development and is involved in plant virulence as assessed in corn and peanut seed-based assays. An antifungal test with the inhibitor azidothymidine suppressed AfNDK activity and prevented spore production and sclerotia formation in , confirming AfNDK's regulatory functions. Crystallographic analysis of AfNDK, coupled with site-directed mutagenesis experiments, revealed three residues (Arg-104, His-117, and Asp-120) as key sites that contribute to spore and sclerotia development. These results not only enrich our knowledge of the regulatory role of this important protein in , but also provide insights into the prevention of infection in plants and seeds, as well as into the structural features relevant for future antifungal drug development.

PubMed: 31243100
DOI: 10.1074/jbc.RA119.007505

Experimental method

X-RAY DIFFRACTION (2.179 Å)