6JZI

Structure of hen egg-white lysozyme obtained from SFX experiments under atmospheric pressure

Summary for 6JZI

• Entry DOI: 10.2210/pdb6jzi/pdb
• Descriptor: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: x-ray free-electron lasers room-temperatur, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 14425.06

Authors

Nango, E.,Sugahara, M.,Nakane, T.,Tanaka, T.,Iwata, S. (deposition date: 2019-05-02, release date: 2019-10-30, Last modification date: 2024-10-16)

Primary citation

Shimazu, Y.,Tono, K.,Tanaka, T.,Yamanaka, Y.,Nakane, T.,Mori, C.,Terakado Kimura, K.,Fujiwara, T.,Sugahara, M.,Tanaka, R.,Doak, R.B.,Shimamura, T.,Iwata, S.,Nango, E.,Yabashi, M.
High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure.
J.Appl.Crystallogr., 52:1280-1288, 2019

PubMed Abstract: A sample-injection device has been developed at SPring-8 Angstrom Compact Free-Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge-type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature-regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A adenosine receptor complexed with an antagonist, ZM241385, in lipidic cubic phase and for hen egg-white lysozyme microcrystals in a grease carrier. The injection device has also been applied to many kinds of proteins, not only for static structural analyses but also for dynamics studies using pump-probe techniques.

PubMed: 31798359
DOI: 10.1107/S1600576719012846

Experimental method

X-RAY DIFFRACTION (2 Å)