6JW8

The crystal structure of KanD2 in complex with NADH and 3"-deamino-3"-hydroxykanamycin B

6JW8 の概要

• エントリーDOI: 10.2210/pdb6jw8/pdb
• 分子名称: Dehydrogenase, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol, ... (4 entities in total)
• 機能のキーワード: rossmann fold, nad binding, oxidoreductase
• 由来する生物種: Streptomyces kanamyceticus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84031.50

構造登録者

Kudo, F.,Kitayama, Y.,Miyanaga, A.,Hirayama, A.,Eguchi, T. (登録日: 2019-04-18, 公開日: 2020-04-15, 最終更新日: 2023-11-22)

• 主引用文献: Kudo, F.,Kitayama, Y.,Miyanaga, A.,Hirayama, A.,Eguchi, T.; Biochemical and structural analysis of a dehydrogenase, KanD2, and an aminotransferase, KanS2, that are responsible for the construction of the kanosamine moiety in kanamycin biosynthesis.; Biochemistry, 59:1470-1473, 2020; PubMed: 32237736; DOI: 10.1021/acs.biochem.0c00204

実験手法

X-RAY DIFFRACTION (2.4 Å)