6JW8

The crystal structure of KanD2 in complex with NADH and 3"-deamino-3"-hydroxykanamycin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Dehydrogenase	polymer	386	41108.1	2	UniProt (Q6L737) Pfam (PF01408) Pfam (PF02894) In PDB	Streptomyces kanamyceticus	KanD2
2	A, B	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	2	Chemie (NAI)
3	A	(2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol	non-polymer		484.5	1	Chemie (CK0)
4		water	water		18.0	30	Chemie (HOH)

Sequence modifications

A, B: 1 - 373 (UniProt: Q6L737)

PDB	External Database	Details
Lys 374	-	expression tag
Leu 375	-	expression tag
Ala 376	-	expression tag
Ala 377	-	expression tag
Ala 378	-	expression tag
Leu 379	-	expression tag
Glu 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82216.1
Non-Polymers*	Number of molecules	3
	Total formula weight	1815.4
All*	Total formula weight	84031.5

*Water molecules are not included.