6JPS

Human H chain ferritin mutant-MBP

6JPS の概要

• エントリーDOI: 10.2210/pdb6jps/pdb
• 分子名称: Ferritin (1 entity in total)
• 機能のキーワード: ferritin, mercuric ion binding peptide, metal binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 522268.87

構造登録者

Wang, Y.,Zang, J.,Chen, H. (登録日: 2019-03-27, 公開日: 2019-10-09, 最終更新日: 2023-11-22)

主引用文献

Wang, Y.,Chen, H.,Zang, J.,Zhang, X.,Zhao, G.
Re-designing ferritin nanocages for mercuric ion detection.
Analyst, 144:5890-5897, 2019

PubMed Abstract: Protein nanocages have recently received considerable attention in the fields of nanoscience and nanomedicine and have been used as either biotemplates for the preparation of a variety of nanomaterials or vehicles for drugs or imaging agents. However, their utilization for detection of heavy metal ions has yet to be explored. In this study, by grafting a mercury binding peptide (MBP) on the exterior surface of a recombinant human H-chain ferritin (rHuHF) nanocage, we successfully prepared a new protein nanocage (HuHF-MBP) which exhibits high binding capacity and affinity for Hg2+. The fluorescence of HuHF-MBP labeled with a green fluorescent dye fluorescein isothiocyanate (FITC) can be quenched by graphene oxide (GO), while addition of Hg2+ to the above solution recovered the quenched fluorescence in a dose-dependent manner. Thus, this system consisting of FITC-labeled HuHF-MBP and GO, where FITC and graphene oxide were used as fluorescent reporter probes, has great potential to be explored as a sensor for Hg2+ detection. Indeed, this newly constructed protein sensor exhibited high sensitivity and selectivity for Hg2+, and the limit of detection was 1.0 nM. The construction of this system provides an alternative strategy for the preparation of heavy metal ion sensors by using protein nanocages as biotemplates.

PubMed: 31497803
DOI: 10.1039/c9an01110b

実験手法

X-RAY DIFFRACTION (3.503 Å)