Summary for 6JNO
| Entry DOI | 10.2210/pdb6jno/pdb |
| Descriptor | Retinoic acid receptor RXR-alpha, 7-oxidanyl-2-oxidanylidene-6-(3,5,5,8,8-pentamethyl-6,7-dihydronaphthalen-2-yl)chromene-3-carboxylic acid (3 entities in total) |
| Functional Keywords | retinoid x receptor alpha, ligand binding domain, fluorescent reagent, inactive form, transcription |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 4 |
| Total formula weight | 108903.64 |
| Authors | Kawasaki, M.,Nakano, S.,Motoyama, T.,Yamada, S.,Watanabe, M.,Takamura, Y.,Fujihara, M.,Tokiwa, H.,Kakuta, H.,Ito, S. (deposition date: 2019-03-17, release date: 2019-11-20, Last modification date: 2024-10-23) |
| Primary citation | Yamada, S.,Kawasaki, M.,Fujihara, M.,Watanabe, M.,Takamura, Y.,Takioku, M.,Nishioka, H.,Takeuchi, Y.,Makishima, M.,Motoyama, T.,Ito, S.,Tokiwa, H.,Nakano, S.,Kakuta, H. Competitive Binding Assay with an Umbelliferone-Based Fluorescent Rexinoid for Retinoid X Receptor Ligand Screening. J.Med.Chem., 62:8809-8818, 2019 Cited by PubMed Abstract: Ligands for retinoid X receptors (RXRs), "rexinoids", are attracting interest as candidates for therapy of type 2 diabetes and Alzheimer's and Parkinson's diseases. However, current screening methods for rexinoids are slow and require special apparatus or facilities. Here, we created 7-hydroxy-2-oxo-6-(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydronaphthalen-2-yl)-2-chromene-3-carboxylic acid (, CU-6PMN) as a new fluorescent RXR agonist and developed a screening system of rexinoids using . Compound was designed based on the fact that umbelliferone emits strong fluorescence in a hydrophilic environment, but the fluorescence intensity decreases in hydrophobic environments such as the interior of proteins. The developed assay using enabled screening of rexinoids to be performed easily within a few hours by monitoring changes of fluorescence intensity with widely available fluorescence microplate readers, without the need for processes such as filtration. PubMed: 31483660DOI: 10.1021/acs.jmedchem.9b00995 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.65 Å) |
Structure validation
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