6JMV

Crystal structure of the GluK3 ligand binding domain complex with SYM and zinc

Summary for 6JMV

• Entry DOI: 10.2210/pdb6jmv/pdb
• Related: 6JFY 6JFZ
• Descriptor: Glutamate receptor ionotropic, kainate 3, 2S,4R-4-METHYLGLUTAMATE, CHLORIDE ION, ... (8 entities in total)
• Functional Keywords: glutamate receptor, kainate, sym, membrane protein
• Biological source: Rattus norvegicus (Rat); More
• Total number of polymer chains: 2
• Total formula weight: 59691.20

Authors

Kumari, J.,Kumar, J. (deposition date: 2019-03-13, release date: 2019-07-24, Last modification date: 2024-10-16)

Primary citation

Kumari, J.,Vinnakota, R.,Kumar, J.
Structural and Functional Insights into GluK3-kainate Receptor Desensitization and Recovery.
Sci Rep, 9:10254-10254, 2019

PubMed Abstract: GluK3-kainate receptors are atypical members of the iGluR family that reside at both the pre- and postsynapse and play a vital role in the regulation of synaptic transmission. For a better understanding of structural changes that underlie receptor functions, GluK3 receptors were trapped in desensitized and resting/closed states and structures analyzed using single particle cryo-electron microscopy. While the desensitized GluK3 has domain organization as seen earlier for another kainate receptor-GluK2, antagonist bound GluK3 trapped a resting state with only two LBD domains in dimeric arrangement necessary for receptor activation. Using structures as a guide, we show that the N-linked glycans at the interface of GluK3 ATD and LBD likely mediate inter-domain interactions and attune receptor-gating properties. The mutational analysis also identified putative N-glycan interacting residues. Our results provide a molecular framework for understanding gating properties unique to GluK3 and exploring the role of N-linked glycosylation in their modulation.

PubMed: 31311973
DOI: 10.1038/s41598-019-46770-z

Experimental method

X-RAY DIFFRACTION (1.832 Å)