6JLY

eIF2a - eIF2B complex

6JLY の概要

• エントリーDOI: 10.2210/pdb6jly/pdb
• 分子名称: Translation initiation factor eIF-2B subunit alpha, Probable translation initiation factor eIF-2B subunit beta, Probable translation initiation factor eIF-2B subunit gamma, ... (7 entities in total)
• 機能のキーワード: translation initiation, translation
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 590952.62

構造登録者

Kashiwagi, K.,Ito, T. (登録日: 2019-03-07, 公開日: 2019-05-01, 最終更新日: 2024-03-27)

主引用文献

Kashiwagi, K.,Yokoyama, T.,Nishimoto, M.,Takahashi, M.,Sakamoto, A.,Yonemochi, M.,Shirouzu, M.,Ito, T.
Structural basis for eIF2B inhibition in integrated stress response.
Science, 364:495-499, 2019

PubMed Abstract: A core event in the integrated stress response, an adaptive pathway common to all eukaryotic cells in response to various stress stimuli, is the phosphorylation of eukaryotic translation initiation factor 2 (eIF2). Normally, unphosphorylated eIF2 transfers the methionylated initiator tRNA to the ribosome in a guanosine 5'-triphosphate-dependent manner. By contrast, phosphorylated eIF2 inhibits its specific guanine nucleotide exchange factor, eIF2B. To elucidate how the eIF2 phosphorylation status regulates the eIF2B activity, we determined cryo-electron microscopic and crystallographic structures of eIF2B in complex with unphosphorylated or phosphorylated eIF2. The unphosphorylated and phosphorylated forms of eIF2 bind to eIF2B in completely different manners: the nucleotide exchange-active and -inactive modes, respectively. These structures explain how phosphorylated eIF2 dominantly inhibits the nucleotide exchange activity of eIF2B.

PubMed: 31048492
DOI: 10.1126/science.aaw4104

実験手法

X-RAY DIFFRACTION (3.5 Å)