6JLM
XFEL structure of cyanobacterial photosystem II (dark state, dataset2)
Summary for 6JLM
| Entry DOI | 10.2210/pdb6jlm/pdb |
| Related | 6JLJ 6JLK 6JLL |
| Descriptor | Photosystem II protein D1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (40 entities in total) |
| Functional Keywords | photosystem ii, photosynthesis, psii, membrane protein |
| Biological source | Thermosynechococcus vulcanus More |
| Total number of polymer chains | 39 |
| Total formula weight | 743079.49 |
| Authors | Suga, M.,Shen, J.R. (deposition date: 2019-03-06, release date: 2019-10-16, Last modification date: 2024-11-06) |
| Primary citation | Suga, M.,Akita, F.,Yamashita, K.,Nakajima, Y.,Ueno, G.,Li, H.,Yamane, T.,Hirata, K.,Umena, Y.,Yonekura, S.,Yu, L.J.,Murakami, H.,Nomura, T.,Kimura, T.,Kubo, M.,Baba, S.,Kumasaka, T.,Tono, K.,Yabashi, M.,Isobe, H.,Yamaguchi, K.,Yamamoto, M.,Ago, H.,Shen, J.R. An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser. Science, 366:334-338, 2019 Cited by PubMed Abstract: Photosynthetic water oxidation is catalyzed by the MnCaO cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S to S). To reveal the mechanism of water oxidation, we analyzed structures of PSII in the S, S, and S states by x-ray free-electron laser serial crystallography. No insertion of water was found in S, but flipping of D1 Glu upon transition to S leads to the opening of a water channel and provides a space for incorporation of an additional oxygen ligand, resulting in an open cubane MnCaO cluster with an oxyl/oxo bridge. Structural changes of PSII between the different S states reveal cooperative action of substrate water access, proton release, and dioxygen formation in photosynthetic water oxidation. PubMed: 31624207DOI: 10.1126/science.aax6998 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.35 Å) |
Structure validation
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