6JK6

Crystal structure of human chitotriosidase-1 (hCHIT) catalytic domain in complex with compound 2-8-s2

6JK6 の概要

• エントリーDOI: 10.2210/pdb6jk6/pdb
• 分子名称: Chitotriosidase-1, 6-azanyl-11-methyl-2-oxidanylidene-7-[[(2R)-oxolan-2-yl]methyl]-N-(pyridin-3-ylmethyl)-1,9-diaza-7-azoniatricyclo[8.4.0.0^{3,8}]tetradeca-3(8),4,6,9,11,13-hexaene-5-carboxamide (3 entities in total)
• 機能のキーワード: chitnase, complex, chitotriosidase, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43559.72

構造登録者

Jiang, X.,Yang, Q. (登録日: 2019-02-27, 公開日: 2020-02-19, 最終更新日: 2024-11-20)

主引用文献

Jiang, X.,Kumar, A.,Motomura, Y.,Liu, T.,Zhou, Y.,Moro, K.,Zhang, K.Y.J.,Yang, Q.
A Series of Compounds Bearing a Dipyrido-Pyrimidine Scaffold Acting as Novel Human and Insect Pest Chitinase Inhibitors.
J.Med.Chem., 63:987-1001, 2020

PubMed Abstract: Chitinases not only play vital roles in the human innate immune system but are also essential for the development of pathogenic fungi and pests. Chitinase inhibitors are efficient tools to investigate the elusive role of human chitinases and to control pathogens and pests. Via hierarchical virtual screening, we have discovered a series of chitinase inhibitors with a novel scaffold that have high inhibitory activities and selectivities against human and insect chitinases. The most potent human chitotriosidase inhibitor, compound , exhibited a of 49 nM, and the most potent inhibitor of the insect pest chitinase Chi-h, compound , exhibited a of 9 nM. The binding of these two most potent inhibitors was confirmed by X-ray crystallography. In a murine model of bleomycin-induced pulmonary fibrosis, compound was found to suppress the chitotriosidase activity by 60%, leading to a significant increase in inflammatory cells and suggesting that chitotriosidase played a protective role.

PubMed: 31928006
DOI: 10.1021/acs.jmedchem.9b01154

実験手法

X-RAY DIFFRACTION (1.571 Å)