6JFI

The symmetric-reconstructed cryo-EM structure of Zika virus-FabZK2B10 complex

6JFI の概要

• エントリーDOI: 10.2210/pdb6jfi/pdb
• 関連するPDBエントリー: 6JEP
• EMDBエントリー: 9811 9812
• 分子名称: ZIKV structural protein E, strutural protein M, FabZK2B10 heavy chain, ... (4 entities in total)
• 機能のキーワード: zikv, antibody, virus
• 由来する生物種: Zika virus; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 284381.62

構造登録者

Wang, L.,Wang, R.K.,Wang, L.,Ben, H.J.,Yu, L.,Gao, F.,Shi, X.L.,Yin, C.B.,Zhang, F.C.,Xiang, Y.,Zhang, L.Q. (登録日: 2019-02-08, 公開日: 2019-04-10, 最終更新日: 2024-03-27)

主引用文献

Wang, L.,Wang, R.,Wang, L.,Ben, H.,Yu, L.,Gao, F.,Shi, X.,Yin, C.,Zhang, F.,Xiang, Y.,Zhang, L.
Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody.
Cell Rep, 26:3360-3368.e5, 2019

PubMed Abstract: We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection and microcephaly in developing mouse embryos. Here, we report the structural features and mechanism of action of ZK2B10. The crystal structure at a resolution of 2.32 Å revealed that the epitope is located on the lateral ridge of DIII of the envelope glycoprotein. Cryo-EM structure with mature ZIKV showed that the antibody binds to DIIIs around the icosahedral 2-fold, 3-fold, and 5-fold axes, a distinct feature compared to those reported for DIII-specific antibodies. The binding of ZK2B10 to ZIKV has no detectable effect on viral attachment to target cells or on conformational changes of the E glycoprotein in the acidic environment, suggesting that ZK2B10 functions at steps between the formation of the fusion intermediate and membrane fusion. These results provide structural and mechanistic insights into how ZK2B10 mediates protection against ZIKV infection.

PubMed: 30893607
DOI: 10.1016/j.celrep.2019.02.062

実験手法

ELECTRON MICROSCOPY (11 Å)