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- EMDB-9812: The symmetric-reconstructed cryo-EM structure of Zika virus-FabZK... -

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Basic information

Entry
Database: EMDB / ID: EMD-9812
TitleThe symmetric-reconstructed cryo-EM structure of Zika virus-FabZK2B10 complex
Map data
Sample
  • Complex: ZIKV complexed with FabZK2B10
    • Protein or peptide: ZIKV structural protein E
    • Protein or peptide: strutural protein M
    • Protein or peptide: FabZK2B10 heavy chain
    • Protein or peptide: FabZK2B10 light chain
KeywordsZIKV / antibody / VIRUS
Function / homology
Function and homology information


response to herbicide / photosystem II / photosynthetic electron transport in photosystem II / chlorophyll binding / plasma membrane-derived thylakoid membrane / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / metal ion binding
Similarity search - Function
Photosystem II protein D1 / Photosynthetic reaction centre, L/M / Photosystem II protein D1/D2 superfamily / Photosynthetic reaction centre protein / Photosynthetic reaction center proteins signature.
Similarity search - Domain/homology
Photosystem q(B) protein
Similarity search - Component
Biological speciesZika virus / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 11.0 Å
AuthorsWang L / Wang RK
CitationJournal: Cell Rep / Year: 2019
Title: Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody.
Authors: Lin Wang / Ruoke Wang / Lei Wang / Haijing Ben / Lei Yu / Fei Gao / Xuanling Shi / Chibiao Yin / Fuchun Zhang / Ye Xiang / Linqi Zhang /
Abstract: We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection and microcephaly in developing mouse embryos. Here, we report the structural ...We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection and microcephaly in developing mouse embryos. Here, we report the structural features and mechanism of action of ZK2B10. The crystal structure at a resolution of 2.32 Å revealed that the epitope is located on the lateral ridge of DIII of the envelope glycoprotein. Cryo-EM structure with mature ZIKV showed that the antibody binds to DIIIs around the icosahedral 2-fold, 3-fold, and 5-fold axes, a distinct feature compared to those reported for DIII-specific antibodies. The binding of ZK2B10 to ZIKV has no detectable effect on viral attachment to target cells or on conformational changes of the E glycoprotein in the acidic environment, suggesting that ZK2B10 functions at steps between the formation of the fusion intermediate and membrane fusion. These results provide structural and mechanistic insights into how ZK2B10 mediates protection against ZIKV infection.
History
DepositionFeb 8, 2019-
Header (metadata) releaseApr 10, 2019-
Map releaseApr 10, 2019-
UpdateMar 27, 2024-
Current statusMar 27, 2024Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3.45
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by radius
  • Surface level: 3.45
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6jfi
  • Surface level: 3.45
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-6jfi
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9812.png

Downloads & links

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Map

FileDownload / File: emd_9812.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.24 Å/pix.
x 320 pix.
= 716.8 Å		2.24 Å/pix.
x 320 pix.
= 716.8 Å		2.24 Å/pix.
x 320 pix.
= 716.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.24 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 3.45 / Movie #1: 3.45
Minimum - Maximum-3.081707 - 6.4863596
Average (Standard dev.)0.2303884 (±1.0)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 716.8 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.242.242.24
M x/y/z320320320
origin x/y/z0.0000.0000.000
length x/y/z716.800716.800716.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS320320320
D min/max/mean-3.0826.4860.230

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Supplemental data

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Sample components

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Entire : ZIKV complexed with FabZK2B10

EntireName: ZIKV complexed with FabZK2B10
Components
  • Complex: ZIKV complexed with FabZK2B10
    • Protein or peptide: ZIKV structural protein E
    • Protein or peptide: strutural protein M
    • Protein or peptide: FabZK2B10 heavy chain
    • Protein or peptide: FabZK2B10 light chain

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Supramolecule #1: ZIKV complexed with FabZK2B10

SupramoleculeName: ZIKV complexed with FabZK2B10 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Zika virus

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Macromolecule #1: ZIKV structural protein E

MacromoleculeName: ZIKV structural protein E / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Zika virus
Molecular weightTheoretical: 54.186762 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: IRCIGVSNRD FVEGMSGGTW VDVVLEHGGC VTVMAQDKPT VDIELVTTTV SNMAEVRSYC YEASISDMAS DSRCPTQGEA YLDKQSDTQ YVCKRTLVDR GWGNGCGLFG KGSLVTCAKF ACSKKMTGKS IQPENLEYRI MLSVHGSQHS GMIVNDTGHE T DENRAKVE ...String:
IRCIGVSNRD FVEGMSGGTW VDVVLEHGGC VTVMAQDKPT VDIELVTTTV SNMAEVRSYC YEASISDMAS DSRCPTQGEA YLDKQSDTQ YVCKRTLVDR GWGNGCGLFG KGSLVTCAKF ACSKKMTGKS IQPENLEYRI MLSVHGSQHS GMIVNDTGHE T DENRAKVE ITPNSPRAEA TLGGFGSLGL DCEPRTGLDF SDLYYLTMNN KHWLVHKEWF HDIPLPWHAG ADTGTPHWNN KE ALVEFKD AHAKRQTVVV LGSQEGAVHT ALAGALEAEM DGAKGRLSSG HLKCRLKMDK LRLKGVSYSL CTAAFTFTKI PAE TLHGTV TVEVQYAGTD GPCKVPAQMA VDMQTLTPVG RLITANPVIT ESTENSKMML ELDPPFGDSY IVIGVGEKKI THHW HRSGS TIGKAFEATV RGAKRMAVLG DTAWDFGSVG GALNSLGKGI HQIFGAAFKS LFGGMSWFSQ ILIGTLLMWL GLNTK NGSI SLMCLALGGV LIFLSTA

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Macromolecule #2: strutural protein M

MacromoleculeName: strutural protein M / type: protein_or_peptide / ID: 2 / Number of copies: 3 / Enantiomer: LEVO / EC number: flavivirin
Source (natural)Organism: Zika virus
Molecular weightTheoretical: 8.496883 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString:
AVTLPSHSTR KLQTRSQTWL ESREYTKHLI RVENWIFRNP GFALAAAAIA WLLGSSTSQK VIYLVMILLI APAYS

UniProtKB: Photosystem q(B) protein

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Macromolecule #3: FabZK2B10 heavy chain

MacromoleculeName: FabZK2B10 heavy chain / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.383305 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: EVQLVQSGAE VKKPGASVKV SCKASGYTFT SGHTFPSYDI NWVRQATGQG LEWMGWMNPN RGNTGYAQKF QGRVTMTRNT SINTAYMEL SSLRSEDTAV YYCARVRSGT NYGSYYYYYY GMDVWGQGTT VTVSSASTKG PSVFPLAPSS KSTSGGTAAL G CLVKDYFP ...String:
EVQLVQSGAE VKKPGASVKV SCKASGYTFT SGHTFPSYDI NWVRQATGQG LEWMGWMNPN RGNTGYAQKF QGRVTMTRNT SINTAYMEL SSLRSEDTAV YYCARVRSGT NYGSYYYYYY GMDVWGQGTT VTVSSASTKG PSVFPLAPSS KSTSGGTAAL G CLVKDYFP EPVTVSWNSG ALTSGVHTFP AVLQSSGLYS LSSVVTVPSS SLGTQTYICN VNHKPSNTKV DKRVEPK

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Macromolecule #4: FabZK2B10 light chain

MacromoleculeName: FabZK2B10 light chain / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.782035 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: SYELTQPPSA SGTPGQRVTI SCSGSSSNIG NNYVHWYQQL PGSAPKLLIY RNNQRPSGVP DRFSGSKSGT SGSLAISGLR SEDEADYYC ASWDDSLSGH WVFGGGTKVT VLGQPKAAPS VTLFPPSSEE LQANKATLVC LISDFYPGAV TVAWKADSSP V KAGVETTT ...String:
SYELTQPPSA SGTPGQRVTI SCSGSSSNIG NNYVHWYQQL PGSAPKLLIY RNNQRPSGVP DRFSGSKSGT SGSLAISGLR SEDEADYYC ASWDDSLSGH WVFGGGTKVT VLGQPKAAPS VTLFPPSSEE LQANKATLVC LISDFYPGAV TVAWKADSSP V KAGVETTT PSKQSNNKYA ASSYLSLTPE QWKSHRSYSC QVTHEGSTVE KTVAPT

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Detector mode: COUNTING / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: I (icosahedral) / Resolution.type: BY AUTHOR / Resolution: 11.0 Å / Resolution method: OTHER / Number images used: 684
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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Atomic model buiding 1

Initial model
PDB IDChain

5ire

chain_id: A, source_name: PDB, initial_model_type: experimental model

5ire

chain_id: B, source_name: PDB, initial_model_type: experimental model

5ire

chain_id: C, source_name: PDB, initial_model_type: experimental model

5ire

chain_id: D, source_name: PDB, initial_model_type: experimental model

5ire

chain_id: E, source_name: PDB, initial_model_type: experimental model

5ire

chain_id: F, source_name: PDB, initial_model_type: experimental model

5jep

chain_id: H, source_name: PDB, initial_model_type: experimental model

5jep

chain_id: L, source_name: PDB, initial_model_type: experimental model
Output model

PDB-6jfi:
The symmetric-reconstructed cryo-EM structure of Zika virus-FabZK2B10 complex

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