6JEP

Structure of a neutralizing antibody bound to the Zika envelope protein domain III

Summary for 6JEP

• Entry DOI: 10.2210/pdb6jep/pdb
• Descriptor: heavy chain of Fab ZK2B10, light chain of Fab ZK2B10, Genome polyprotein, ... (4 entities in total)
• Functional Keywords: antibody, complex, antiviral protein
• Biological source: Homo sapiens; More
• Total number of polymer chains: 6
• Total formula weight: 118309.81

Authors

Wang, L.,Wang, R.K.,Wang, L.,Ben, H.J.,Yu, L.,Gao, F.,Shi, X.L.,Yin, C.B.,Zhang, F.C.,Xiang, Y.,Zhang, L.Q. (deposition date: 2019-02-07, release date: 2019-05-15, Last modification date: 2024-10-23)

Primary citation

Wang, L.,Wang, R.,Wang, L.,Ben, H.,Yu, L.,Gao, F.,Shi, X.,Yin, C.,Zhang, F.,Xiang, Y.,Zhang, L.
Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody.
Cell Rep, 26:3360-3368.e5, 2019

PubMed Abstract: We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection and microcephaly in developing mouse embryos. Here, we report the structural features and mechanism of action of ZK2B10. The crystal structure at a resolution of 2.32 Å revealed that the epitope is located on the lateral ridge of DIII of the envelope glycoprotein. Cryo-EM structure with mature ZIKV showed that the antibody binds to DIIIs around the icosahedral 2-fold, 3-fold, and 5-fold axes, a distinct feature compared to those reported for DIII-specific antibodies. The binding of ZK2B10 to ZIKV has no detectable effect on viral attachment to target cells or on conformational changes of the E glycoprotein in the acidic environment, suggesting that ZK2B10 functions at steps between the formation of the fusion intermediate and membrane fusion. These results provide structural and mechanistic insights into how ZK2B10 mediates protection against ZIKV infection.

PubMed: 30893607
DOI: 10.1016/j.celrep.2019.02.062

Experimental method

X-RAY DIFFRACTION (2.316 Å)