6JF2

Crystal structure of a 20kDa fragment of FlgG

Summary for 6JF2

• Entry DOI: 10.2210/pdb6jf2/pdb
• Related: 5wrh
• Descriptor: Flagellar basal-body rod protein FlgG (2 entities in total)
• Functional Keywords: bacterial flagellum, distal rod, structural protein
• Biological source: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
• Total number of polymer chains: 2
• Total formula weight: 38771.47

Authors

Saijo-Hamano, Y.,Matsunami, H.,Namba, K.,Imada, K. (deposition date: 2019-02-08, release date: 2019-07-17, Last modification date: 2024-10-30)

Primary citation

Saijo-Hamano, Y.,Matsunami, H.,Namba, K.,Imada, K.
Architecture of the Bacterial Flagellar Distal Rod and Hook of Salmonella .
Biomolecules, 9:-, 2019

PubMed Abstract: The bacterial flagellum is a large molecular complex composed of thousands of protein subunits for motility. The filamentous part of the flagellum, which is called the axial structure, consists of the filament, the hook, and the rods, with other minor components-the cap protein and the hook associated proteins. They share a common basic architecture of subunit arrangement, but each part shows quite distinct mechanical properties to achieve its specific function. The distal rod and the hook are helical assemblies of a single protein, FlgG and FlgE, respectively. They show a significant sequence similarity but have distinct mechanical characteristics. The rod is a rigid, straight cylinder, whereas the hook is a curved tube with high bending flexibility. Here, we report a structural model of the rod constructed by using the crystal structure of a core fragment of FlgG with a density map obtained previously by electron cryomicroscopy. Our structural model suggests that a segment called L-stretch plays a key role in achieving the distinct mechanical properties of the rod using a structurally similar component protein to that of the hook.

PubMed: 31284631
DOI: 10.3390/biom9070260

Experimental method

X-RAY DIFFRACTION (2 Å)