6JDV

Crystal structure of Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in catalytic state

6JDV の概要

• エントリーDOI: 10.2210/pdb6jdv/pdb
• 分子名称: CRISPR-associated endonuclease Cas9, sgRNA, target DNA strand, ... (7 entities in total)
• 機能のキーワード: crispr-cas9, nmecas9, nme1cas9, hydrolase, ternary complex, hydrolase-rna-dna complex, hydrolase/rna/dna
• 由来する生物種: Neisseria meningitidis serogroup C (strain 8013); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 185820.16

構造登録者

Sun, W.,Yang, J.,Cheng, Z.,Liu, C.,Wang, K.,Huang, X.,Wang, Y. (登録日: 2019-02-02, 公開日: 2019-11-06, 最終更新日: 2023-11-22)

• 主引用文献: Sun, W.,Yang, J.,Cheng, Z.,Amrani, N.,Liu, C.,Wang, K.,Ibraheim, R.,Edraki, A.,Huang, X.,Wang, M.,Wang, J.,Liu, L.,Sheng, G.,Yang, Y.,Lou, J.,Sontheimer, E.J.,Wang, Y.; Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.; Mol.Cell, 76:938-, 2019; PubMed: 31668930; DOI: 10.1016/j.molcel.2019.09.025

実験手法

X-RAY DIFFRACTION (3.1 Å)