6JD9

Proteus mirabilis lipase mutant - I118V/E130G

Summary for 6JD9

• Entry DOI: 10.2210/pdb6jd9/pdb
• Descriptor: Alpha/beta hydrolase, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• Functional Keywords: lipase, proteus mirabilis lipase, hydrolase
• Biological source: Proteus mirabilis
• Total number of polymer chains: 1
• Total formula weight: 31738.79

Authors

Heater, B.S.,Chan, W.S.,Chan, M.K. (deposition date: 2019-01-31, release date: 2019-07-24, Last modification date: 2024-03-27)

Primary citation

Heater, B.S.,Chan, W.S.,Lee, M.M.,Chan, M.K.
Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil.
Biotechnol Biofuels, 12:165-165, 2019

PubMed Abstract: We have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of the fusion crystals from . Using this approach, lipase A was genetically fused to Cry3Aa to produce a Cry3Aa-lipA catalyst capable of the facile conversion of coconut oil into biodiesel over 10 reaction cycles. Here, we investigate the fusion of another lipase to Cry3Aa with the goal of producing a catalyst suitable for the conversion of waste cooking oil into biodiesel.

PubMed: 31297153
DOI: 10.1186/s13068-019-1509-5

Experimental method

X-RAY DIFFRACTION (1.58 Å)