6JCE
NMR solution and X-ray crystal structures of a DNA containing both right-and left-handed parallel-stranded G-quadruplexes
Summary for 6JCE
| Entry DOI | 10.2210/pdb6jce/pdb |
| Descriptor | 29-mer DNA (1 entity in total) |
| Functional Keywords | left-handed, z-g4, g-quadruplex, hybrid, dna |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 9201.85 |
| Authors | Winnerdy, F.R.,Bakalar, B.,Maity, A.,Vandana, J.J.,Mechulam, Y.,Schmitt, E.,Phan, A.T. (deposition date: 2019-01-28, release date: 2019-07-10, Last modification date: 2024-05-15) |
| Primary citation | Winnerdy, F.R.,Bakalar, B.,Maity, A.,Vandana, J.J.,Mechulam, Y.,Schmitt, E.,Phan, A.T. NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes. Nucleic Acids Res., 47:8272-8281, 2019 Cited by PubMed Abstract: Analogous to the B- and Z-DNA structures in double-helix DNA, there exist both right- and left-handed quadruple-helix (G-quadruplex) DNA. Numerous conformations of right-handed and a few left-handed G-quadruplexes were previously observed, yet they were always identified separately. Here, we present the NMR solution and X-ray crystal structures of a right- and left-handed hybrid G-quadruplex. The structure reveals a stacking interaction between two G-quadruplex blocks with different helical orientations and displays features of both right- and left-handed G-quadruplexes. An analysis of loop mutations suggests that single-nucleotide loops are preferred or even required for the left-handed G-quadruplex formation. The discovery of a right- and left-handed hybrid G-quadruplex further expands the polymorphism of G-quadruplexes and is potentially useful in designing a left-to-right junction in G-quadruplex engineering. PubMed: 31216034DOI: 10.1093/nar/gkz349 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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